Plasmid

Part:BBa_J61148:Design

Designed by: John Anderson   Group: Arkin Lab   (2007-09-04)


pBca9145 basic pUC/Amp plasmid in BBb


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2058
    Illegal PstI site found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2058
    Illegal PstI site found at 16
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 2058
    Illegal suffix found in sequence at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2058
    Illegal PstI site found at 16
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2058
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1097


Design Notes


This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.


Source

pSB1A2, synthetic

References